{"paper":{"title":"Angstrom-resolution single-molecule fluorescence resonance energy transfer reveals mechanisms of DNA helicases","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["q-bio.BM"],"primary_cat":"physics.bio-ph","authors_text":"Bo Zhang, Chunhua Xu, Daguan Nong, Jianbing Ma, Jinghua Li, Ming Li, Qi Jia, Shuoxing Dou, Wenxia Lin, Xuguang Xi, Ying Lu","submitted_at":"2017-04-05T11:18:01Z","abstract_excerpt":"Single-molecule FRET is widely used to study helicases by detecting distance changes between a fluorescent donor and an acceptor anchored to overhangs of a forked DNA duplex. However, it has lacked single-base pair (1-bp) resolution required for revealing stepping dynamics in unwinding because FRET signals are usually blurred by thermal fluctuations of the overhangs. We designed a nanotensioner in which a short DNA is bent to exert a force on the overhangs, just as in optical/magnetic tweezers. The strategy improved the resolution of FRET to 0.5 bp, high enough to uncover the differences in DN"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1704.01369","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}