{"paper":{"title":"Probing single protein dynamics on liposome surfaces","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["q-bio.BM"],"primary_cat":"physics.bio-ph","authors_text":"Chun-Hua Xu, Chun-Yu Zhao, Cong Liu, Dong-Fei Ma, Jiajie Diao, Lu Ma, Ming Li, Wen-Qing Hou, Ying Lu","submitted_at":"2018-05-28T09:38:48Z","abstract_excerpt":"It is crucial to measure position and conformational changes of a membrane-interacting protein relative to the membrane surface. This is however challenging because the thickness of a membrane is usually only about 4 nm. We developed a fluorescence method which makes use of the principle of FRET between a fluorophore and a cloud of quenchers encapsulated in a liposome, hence the name LipoFRET. LipoFRET can readily locate a fluorophore in different depths inside and at different heights above the membrane. We applied LipoFRET to study {\\alpha}-synuclein, a key player in the pathology of Parkins"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1805.10840","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}