{"paper":{"title":"Volumetric nanoscale localization using engineered point spread functions in light sheet microscopy","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":[],"primary_cat":"physics.optics","authors_text":"A. Upadhya, D. J. X. Chow, K. Dholakia, K. R. Dunning, R. E. Bautista Gonzalez, R. Mouthaan","submitted_at":"2026-06-17T07:55:07Z","abstract_excerpt":"Nanoscale three-dimensional localization across large biological volumes remains an outstanding challenge in optical microscopy, with existing approaches typically limited by imaging speed, volumetric field of view and localization precision when required simultaneously. Here, we overcome these limitations by combining a twin Airy engineered point spread function with two-photon light sheet fluorescence microscopy, enabling nanoscale localization throughout large volumetric fields of view. Our framework explicitly incorporates the broadband fluorescence emission characteristic of biological fl"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2606.18784","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2606.18784/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}