{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2012:KVDW7LY5FZFEOQTDGGGHS7RHMC","short_pith_number":"pith:KVDW7LY5","canonical_record":{"source":{"id":"1211.6065","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2012-11-26T19:12:36Z","cross_cats_sorted":[],"title_canon_sha256":"3e94525902a5b6c91a418114bbc075294ee95053d25ec47deef3b809f18bba1f","abstract_canon_sha256":"f9937429f50bb80a33930d33f1faf87cc07aef74edf6b4cc515b274fcab36ada"},"schema_version":"1.0"},"canonical_sha256":"55476faf1d2e4a474263318c797e2760af50feee51f17c1515b76572e7333751","source":{"kind":"arxiv","id":"1211.6065","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1211.6065","created_at":"2026-05-18T03:33:13Z"},{"alias_kind":"arxiv_version","alias_value":"1211.6065v1","created_at":"2026-05-18T03:33:13Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1211.6065","created_at":"2026-05-18T03:33:13Z"},{"alias_kind":"pith_short_12","alias_value":"KVDW7LY5FZFE","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_16","alias_value":"KVDW7LY5FZFEOQTD","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_8","alias_value":"KVDW7LY5","created_at":"2026-05-18T12:27:11Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2012:KVDW7LY5FZFEOQTDGGGHS7RHMC","target":"record","payload":{"canonical_record":{"source":{"id":"1211.6065","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2012-11-26T19:12:36Z","cross_cats_sorted":[],"title_canon_sha256":"3e94525902a5b6c91a418114bbc075294ee95053d25ec47deef3b809f18bba1f","abstract_canon_sha256":"f9937429f50bb80a33930d33f1faf87cc07aef74edf6b4cc515b274fcab36ada"},"schema_version":"1.0"},"canonical_sha256":"55476faf1d2e4a474263318c797e2760af50feee51f17c1515b76572e7333751","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T03:33:13.667682Z","signature_b64":"OROdCNg4QYPfKVIbNljdJlNEPoqe/ZB1QOS4FfWcUC0az7nv5iwZ/zKmQ87nVhyxh3ad31d77kbvY4jNLWBEDA==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"55476faf1d2e4a474263318c797e2760af50feee51f17c1515b76572e7333751","last_reissued_at":"2026-05-18T03:33:13.666669Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T03:33:13.666669Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1211.6065","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:33:13Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"h4nU+GAExy3l30VBvrrOqQ5dnD7EAANs7wcFQqElz2uJvZfYM8077Ssd5jZCpcVtDuOtvhGu9HVTAu8T7EHQCg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-07-02T05:40:28.788856Z"},"content_sha256":"879eb040d4b2e6f427af4b5bbcfcf249808187c491b5d3833edaf37c0246f5cf","schema_version":"1.0","event_id":"sha256:879eb040d4b2e6f427af4b5bbcfcf249808187c491b5d3833edaf37c0246f5cf"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2012:KVDW7LY5FZFEOQTDGGGHS7RHMC","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Mechanical probes of SOD1 predict systematic trends in metal and dimer affinity of ALS-associated mutants","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.BM","authors_text":"Atanu Das, Steven S. Plotkin","submitted_at":"2012-11-26T19:12:36Z","abstract_excerpt":"Mutations and oxidative modification in the protein Cu,Zn superoxide dismutase (SOD1) have been implicated in the death of motor neurons in amyotrophic lateral sclerosis (ALS), a presently incurable, invariably fatal neurodegenerative disease. Here we employ steered, all-atom molecular dynamics simulations in implicit solvent to investigate the significance of either mutations or post-translational modifications (PTMs) to SOD1 on metal affinity, dimer stability, and mechanical malleability. The work required to induce moderate structural deformations as a function of sequence index constitutes"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1211.6065","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:33:13Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"evQPggrv9M7aG+GK0zeTPoa2nz4EXDlKCPLFk1Be1u3z/1mjfXC4PW3fkzFs7oa/I5tzF34p9ff1avOzFFfJCA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-07-02T05:40:28.789195Z"},"content_sha256":"574230426a7bb9975c09002abbba5f45356e6054ee44305b4f3eb541ac5584dc","schema_version":"1.0","event_id":"sha256:574230426a7bb9975c09002abbba5f45356e6054ee44305b4f3eb541ac5584dc"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/KVDW7LY5FZFEOQTDGGGHS7RHMC/bundle.json","state_url":"https://pith.science/pith/KVDW7LY5FZFEOQTDGGGHS7RHMC/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/KVDW7LY5FZFEOQTDGGGHS7RHMC/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-07-02T05:40:28Z","links":{"resolver":"https://pith.science/pith/KVDW7LY5FZFEOQTDGGGHS7RHMC","bundle":"https://pith.science/pith/KVDW7LY5FZFEOQTDGGGHS7RHMC/bundle.json","state":"https://pith.science/pith/KVDW7LY5FZFEOQTDGGGHS7RHMC/state.json","well_known_bundle":"https://pith.science/.well-known/pith/KVDW7LY5FZFEOQTDGGGHS7RHMC/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2012:KVDW7LY5FZFEOQTDGGGHS7RHMC","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"f9937429f50bb80a33930d33f1faf87cc07aef74edf6b4cc515b274fcab36ada","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2012-11-26T19:12:36Z","title_canon_sha256":"3e94525902a5b6c91a418114bbc075294ee95053d25ec47deef3b809f18bba1f"},"schema_version":"1.0","source":{"id":"1211.6065","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1211.6065","created_at":"2026-05-18T03:33:13Z"},{"alias_kind":"arxiv_version","alias_value":"1211.6065v1","created_at":"2026-05-18T03:33:13Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1211.6065","created_at":"2026-05-18T03:33:13Z"},{"alias_kind":"pith_short_12","alias_value":"KVDW7LY5FZFE","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_16","alias_value":"KVDW7LY5FZFEOQTD","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_8","alias_value":"KVDW7LY5","created_at":"2026-05-18T12:27:11Z"}],"graph_snapshots":[{"event_id":"sha256:574230426a7bb9975c09002abbba5f45356e6054ee44305b4f3eb541ac5584dc","target":"graph","created_at":"2026-05-18T03:33:13Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"Mutations and oxidative modification in the protein Cu,Zn superoxide dismutase (SOD1) have been implicated in the death of motor neurons in amyotrophic lateral sclerosis (ALS), a presently incurable, invariably fatal neurodegenerative disease. Here we employ steered, all-atom molecular dynamics simulations in implicit solvent to investigate the significance of either mutations or post-translational modifications (PTMs) to SOD1 on metal affinity, dimer stability, and mechanical malleability. The work required to induce moderate structural deformations as a function of sequence index constitutes","authors_text":"Atanu Das, Steven S. Plotkin","cross_cats":[],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2012-11-26T19:12:36Z","title":"Mechanical probes of SOD1 predict systematic trends in metal and dimer affinity of ALS-associated mutants"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1211.6065","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:879eb040d4b2e6f427af4b5bbcfcf249808187c491b5d3833edaf37c0246f5cf","target":"record","created_at":"2026-05-18T03:33:13Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"f9937429f50bb80a33930d33f1faf87cc07aef74edf6b4cc515b274fcab36ada","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2012-11-26T19:12:36Z","title_canon_sha256":"3e94525902a5b6c91a418114bbc075294ee95053d25ec47deef3b809f18bba1f"},"schema_version":"1.0","source":{"id":"1211.6065","kind":"arxiv","version":1}},"canonical_sha256":"55476faf1d2e4a474263318c797e2760af50feee51f17c1515b76572e7333751","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"55476faf1d2e4a474263318c797e2760af50feee51f17c1515b76572e7333751","first_computed_at":"2026-05-18T03:33:13.666669Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T03:33:13.666669Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"OROdCNg4QYPfKVIbNljdJlNEPoqe/ZB1QOS4FfWcUC0az7nv5iwZ/zKmQ87nVhyxh3ad31d77kbvY4jNLWBEDA==","signature_status":"signed_v1","signed_at":"2026-05-18T03:33:13.667682Z","signed_message":"canonical_sha256_bytes"},"source_id":"1211.6065","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:879eb040d4b2e6f427af4b5bbcfcf249808187c491b5d3833edaf37c0246f5cf","sha256:574230426a7bb9975c09002abbba5f45356e6054ee44305b4f3eb541ac5584dc"],"state_sha256":"32dc047c9bdfa0c711591e63fe9d4cb9c07e9a4fc46cf9e4ee36735282e1eff8"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"/EDBONXnt1p6oYcf6pyP++UPH0cbMk2minfbZBGqJwMLDtf6c0Vgps4K/PUik2ozlnogh5L29+7q96Z6jJWhCg==","signed_message":"bundle_sha256_bytes","signed_at":"2026-07-02T05:40:28.791346Z","bundle_sha256":"1605eb691b22e9bfd967853b61cb215a17a8f3ad68933552603310117ee6cce8"}}