{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2012:LQI5GPWJFRMNI6ZSEFHRHCBXTV","short_pith_number":"pith:LQI5GPWJ","schema_version":"1.0","canonical_sha256":"5c11d33ec92c58d47b32214f1388379d741b533220e1ebc8c525cce867172357","source":{"kind":"arxiv","id":"1210.4938","version":1},"attestation_state":"computed","paper":{"title":"Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\\\"id cell lines nuclear proteomes","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.GN","authors_text":"Alain van Dorsselaer (IPHC-DSA), C\\'ecile Lelong (LCBM), H\\'el\\`ene Diemer (IPHC-DSA), Mireille Chevallet (LCBM), Sylvie Luche (LCBM), Thierry Rabilloud (LCBM)","submitted_at":"2012-10-17T20:02:51Z","abstract_excerpt":"One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids. Several experimental procedures have been tested to increase, in the nuclear extract, the ratio of nuclear proteins compared to contaminant proteins, and also to obtain reproducible conditions compatible with 2D-gel electrophoresis. The NaCl procedure has been chosen. To test the interest of this procedure, the nuclear protein expression profiles of macro"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"1210.4938","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-10-17T20:02:51Z","cross_cats_sorted":[],"title_canon_sha256":"f3231e65a94e8fe30d8424e0d4bd4188c68af2ed496180f23475287bfe2a485c","abstract_canon_sha256":"c05634645e293651a31d36d13eebcda9c70b93955e14a57069ba05c589caca2f"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T03:42:54.856779Z","signature_b64":"B9l3bjNIHv5C6twvSmI/BsWNrDjKrWeQZcTLNtqCiq6LU4kVfcvu8LEEKHPe4Ty8WLlEw97C3sAg/d2/QBwzBQ==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"5c11d33ec92c58d47b32214f1388379d741b533220e1ebc8c525cce867172357","last_reissued_at":"2026-05-18T03:42:54.856134Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T03:42:54.856134Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myelo\\\"id cell lines nuclear proteomes","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.GN","authors_text":"Alain van Dorsselaer (IPHC-DSA), C\\'ecile Lelong (LCBM), H\\'el\\`ene Diemer (IPHC-DSA), Mireille Chevallet (LCBM), Sylvie Luche (LCBM), Thierry Rabilloud (LCBM)","submitted_at":"2012-10-17T20:02:51Z","abstract_excerpt":"One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids. Several experimental procedures have been tested to increase, in the nuclear extract, the ratio of nuclear proteins compared to contaminant proteins, and also to obtain reproducible conditions compatible with 2D-gel electrophoresis. The NaCl procedure has been chosen. To test the interest of this procedure, the nuclear protein expression profiles of macro"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1210.4938","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"1210.4938","created_at":"2026-05-18T03:42:54.856220+00:00"},{"alias_kind":"arxiv_version","alias_value":"1210.4938v1","created_at":"2026-05-18T03:42:54.856220+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1210.4938","created_at":"2026-05-18T03:42:54.856220+00:00"},{"alias_kind":"pith_short_12","alias_value":"LQI5GPWJFRMN","created_at":"2026-05-18T12:27:14.488303+00:00"},{"alias_kind":"pith_short_16","alias_value":"LQI5GPWJFRMNI6ZS","created_at":"2026-05-18T12:27:14.488303+00:00"},{"alias_kind":"pith_short_8","alias_value":"LQI5GPWJ","created_at":"2026-05-18T12:27:14.488303+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV","json":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV.json","graph_json":"https://pith.science/api/pith-number/LQI5GPWJFRMNI6ZSEFHRHCBXTV/graph.json","events_json":"https://pith.science/api/pith-number/LQI5GPWJFRMNI6ZSEFHRHCBXTV/events.json","paper":"https://pith.science/paper/LQI5GPWJ"},"agent_actions":{"view_html":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV","download_json":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV.json","view_paper":"https://pith.science/paper/LQI5GPWJ","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=1210.4938&json=true","fetch_graph":"https://pith.science/api/pith-number/LQI5GPWJFRMNI6ZSEFHRHCBXTV/graph.json","fetch_events":"https://pith.science/api/pith-number/LQI5GPWJFRMNI6ZSEFHRHCBXTV/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV/action/timestamp_anchor","attest_storage":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV/action/storage_attestation","attest_author":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV/action/author_attestation","sign_citation":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV/action/citation_signature","submit_replication":"https://pith.science/pith/LQI5GPWJFRMNI6ZSEFHRHCBXTV/action/replication_record"}},"created_at":"2026-05-18T03:42:54.856220+00:00","updated_at":"2026-05-18T03:42:54.856220+00:00"}