{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2014:TQOHHXUDQGDJE3C3JIFOMKGWFB","short_pith_number":"pith:TQOHHXUD","schema_version":"1.0","canonical_sha256":"9c1c73de838186926c5b4a0ae628d6287efc915b9099e4ccc2c609d1af5fe946","source":{"kind":"arxiv","id":"1403.3066","version":4},"attestation_state":"computed","paper":{"title":"Quantifying evolutionary constraints on B cell affinity maturation","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.PE","authors_text":"Connor O. McCoy, Frederick A. Matsen IV, Harlan Robins, Philip Bradley, Trevor Bedford, Vladimir N. Minin","submitted_at":"2014-03-12T19:01:41Z","abstract_excerpt":"The antibody repertoire of each individual is continuously updated by the evolutionary process of B cell receptor mutation and selection. It has recently become possible to gain detailed information concerning this process through high-throughput sequencing. Here, we develop modern statistical molecular evolution methods for the analysis of B cell sequence data, and then apply them to a very deep short-read data set of B cell receptors. We find that the substitution process is conserved across individuals but varies significantly across gene segments. We investigate selection on B cell recepto"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"1403.3066","kind":"arxiv","version":4},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.PE","submitted_at":"2014-03-12T19:01:41Z","cross_cats_sorted":[],"title_canon_sha256":"9f9dcb03938f4bec0c95cacac97ccd4d396abeae49c82cea96ea2dd41eab58f1","abstract_canon_sha256":"0cb6745ca477423c88bcb70f6618131bce4b0125e1e536791723d0ec2445f184"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T02:16:39.395975Z","signature_b64":"GqcRrncXLrWLU42Vd2Rr8JRg+S7HBGYLFHaWAtkNWmp6vOtpsAJ6XiYX+vw2rePgBCL5lAKzB72rFaxYiSdEDg==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"9c1c73de838186926c5b4a0ae628d6287efc915b9099e4ccc2c609d1af5fe946","last_reissued_at":"2026-05-18T02:16:39.395513Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T02:16:39.395513Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"Quantifying evolutionary constraints on B cell affinity maturation","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.PE","authors_text":"Connor O. McCoy, Frederick A. Matsen IV, Harlan Robins, Philip Bradley, Trevor Bedford, Vladimir N. Minin","submitted_at":"2014-03-12T19:01:41Z","abstract_excerpt":"The antibody repertoire of each individual is continuously updated by the evolutionary process of B cell receptor mutation and selection. It has recently become possible to gain detailed information concerning this process through high-throughput sequencing. Here, we develop modern statistical molecular evolution methods for the analysis of B cell sequence data, and then apply them to a very deep short-read data set of B cell receptors. We find that the substitution process is conserved across individuals but varies significantly across gene segments. We investigate selection on B cell recepto"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1403.3066","kind":"arxiv","version":4},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"1403.3066","created_at":"2026-05-18T02:16:39.395581+00:00"},{"alias_kind":"arxiv_version","alias_value":"1403.3066v4","created_at":"2026-05-18T02:16:39.395581+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1403.3066","created_at":"2026-05-18T02:16:39.395581+00:00"},{"alias_kind":"pith_short_12","alias_value":"TQOHHXUDQGDJ","created_at":"2026-05-18T12:28:49.207871+00:00"},{"alias_kind":"pith_short_16","alias_value":"TQOHHXUDQGDJE3C3","created_at":"2026-05-18T12:28:49.207871+00:00"},{"alias_kind":"pith_short_8","alias_value":"TQOHHXUD","created_at":"2026-05-18T12:28:49.207871+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB","json":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB.json","graph_json":"https://pith.science/api/pith-number/TQOHHXUDQGDJE3C3JIFOMKGWFB/graph.json","events_json":"https://pith.science/api/pith-number/TQOHHXUDQGDJE3C3JIFOMKGWFB/events.json","paper":"https://pith.science/paper/TQOHHXUD"},"agent_actions":{"view_html":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB","download_json":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB.json","view_paper":"https://pith.science/paper/TQOHHXUD","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=1403.3066&json=true","fetch_graph":"https://pith.science/api/pith-number/TQOHHXUDQGDJE3C3JIFOMKGWFB/graph.json","fetch_events":"https://pith.science/api/pith-number/TQOHHXUDQGDJE3C3JIFOMKGWFB/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB/action/timestamp_anchor","attest_storage":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB/action/storage_attestation","attest_author":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB/action/author_attestation","sign_citation":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB/action/citation_signature","submit_replication":"https://pith.science/pith/TQOHHXUDQGDJE3C3JIFOMKGWFB/action/replication_record"}},"created_at":"2026-05-18T02:16:39.395581+00:00","updated_at":"2026-05-18T02:16:39.395581+00:00"}