{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2010:ZJ3P46E3A7BL5TQDC2WIXWAZIT","short_pith_number":"pith:ZJ3P46E3","canonical_record":{"source":{"id":"1012.4313","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2010-12-20T12:58:55Z","cross_cats_sorted":[],"title_canon_sha256":"502dbcde5e5bc74f92bc21af1923258c1f5e7214edcd6eeac0036ef757c8b94c","abstract_canon_sha256":"1cdb862e2ec31e9a0e7d4192680193a714eaf88bbbf47728ac06c22288e7770b"},"schema_version":"1.0"},"canonical_sha256":"ca76fe789b07c2bece0316ac8bd81944c1a5e9eec2002318e14b192b0f1140bf","source":{"kind":"arxiv","id":"1012.4313","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1012.4313","created_at":"2026-05-18T04:32:55Z"},{"alias_kind":"arxiv_version","alias_value":"1012.4313v1","created_at":"2026-05-18T04:32:55Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1012.4313","created_at":"2026-05-18T04:32:55Z"},{"alias_kind":"pith_short_12","alias_value":"ZJ3P46E3A7BL","created_at":"2026-05-18T12:26:17Z"},{"alias_kind":"pith_short_16","alias_value":"ZJ3P46E3A7BL5TQD","created_at":"2026-05-18T12:26:17Z"},{"alias_kind":"pith_short_8","alias_value":"ZJ3P46E3","created_at":"2026-05-18T12:26:17Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2010:ZJ3P46E3A7BL5TQDC2WIXWAZIT","target":"record","payload":{"canonical_record":{"source":{"id":"1012.4313","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2010-12-20T12:58:55Z","cross_cats_sorted":[],"title_canon_sha256":"502dbcde5e5bc74f92bc21af1923258c1f5e7214edcd6eeac0036ef757c8b94c","abstract_canon_sha256":"1cdb862e2ec31e9a0e7d4192680193a714eaf88bbbf47728ac06c22288e7770b"},"schema_version":"1.0"},"canonical_sha256":"ca76fe789b07c2bece0316ac8bd81944c1a5e9eec2002318e14b192b0f1140bf","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T04:32:55.032751Z","signature_b64":"eYVrqwVGGkCyo11LoiuPEmBEZ/dmPmgeE4c+Nn9InB58J+dj0jI7ilfMzwdurunIGx/puf872KinTrwLmLmNCA==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"ca76fe789b07c2bece0316ac8bd81944c1a5e9eec2002318e14b192b0f1140bf","last_reissued_at":"2026-05-18T04:32:55.032054Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T04:32:55.032054Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1012.4313","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T04:32:55Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"q6qiZQ6QL3blE6eki8Dr/5Y+bYXplywNUrADgQzRsMwJZa2SNWzp7pkWYyeNMvwx1dRAChNLhLhAKhvvmInYAg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-07-02T13:22:59.528474Z"},"content_sha256":"5fba30d2485d130ce1c93d9bf687985f7fcffe8b8b3cbbe9311ed382e0971804","schema_version":"1.0","event_id":"sha256:5fba30d2485d130ce1c93d9bf687985f7fcffe8b8b3cbbe9311ed382e0971804"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2010:ZJ3P46E3A7BL5TQDC2WIXWAZIT","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Exploring dimethylsulfate for in vivo proteome footprinting","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.MN","authors_text":"Arman Kulyyassov, Damien Vertut, Erlan Ramankulov, Luc Camoin, Marc Lipinski, Pasquale Moio, Vasily Ogryzko","submitted_at":"2010-12-20T12:58:55Z","abstract_excerpt":"Protein footprinting is a new methodology that is based on probing, typically with the use of mass spectrometry, of reactivity of different aminoacid residues to a modifying reagent. Data thus obtained allow one to make inferences about protein conformations and their intermolecular interactions. Most of the protein footprinting studies so far have been performed on individual proteins in vitro. We explore whether a similar approach is possible with the proteins inside of living cells, employing dimethylsulfate (DMS), a reagent widely used for the in vivo footprinting of nucleic acids. DMS can"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1012.4313","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T04:32:55Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"Hfk8bNwkI+JHPwatAUlSvUVsNV/sWXJH/Xt8CFbix9qOs1N8htiyIv/OMX2mWsIXqQaQf4ODR+RWET2jCiA6Aw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-07-02T13:22:59.528819Z"},"content_sha256":"69d3a1e3e284a60919d2d9447332cf8df3f8dc34efec3371adc439e7081d3958","schema_version":"1.0","event_id":"sha256:69d3a1e3e284a60919d2d9447332cf8df3f8dc34efec3371adc439e7081d3958"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/ZJ3P46E3A7BL5TQDC2WIXWAZIT/bundle.json","state_url":"https://pith.science/pith/ZJ3P46E3A7BL5TQDC2WIXWAZIT/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/ZJ3P46E3A7BL5TQDC2WIXWAZIT/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-07-02T13:22:59Z","links":{"resolver":"https://pith.science/pith/ZJ3P46E3A7BL5TQDC2WIXWAZIT","bundle":"https://pith.science/pith/ZJ3P46E3A7BL5TQDC2WIXWAZIT/bundle.json","state":"https://pith.science/pith/ZJ3P46E3A7BL5TQDC2WIXWAZIT/state.json","well_known_bundle":"https://pith.science/.well-known/pith/ZJ3P46E3A7BL5TQDC2WIXWAZIT/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2010:ZJ3P46E3A7BL5TQDC2WIXWAZIT","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"1cdb862e2ec31e9a0e7d4192680193a714eaf88bbbf47728ac06c22288e7770b","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2010-12-20T12:58:55Z","title_canon_sha256":"502dbcde5e5bc74f92bc21af1923258c1f5e7214edcd6eeac0036ef757c8b94c"},"schema_version":"1.0","source":{"id":"1012.4313","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1012.4313","created_at":"2026-05-18T04:32:55Z"},{"alias_kind":"arxiv_version","alias_value":"1012.4313v1","created_at":"2026-05-18T04:32:55Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1012.4313","created_at":"2026-05-18T04:32:55Z"},{"alias_kind":"pith_short_12","alias_value":"ZJ3P46E3A7BL","created_at":"2026-05-18T12:26:17Z"},{"alias_kind":"pith_short_16","alias_value":"ZJ3P46E3A7BL5TQD","created_at":"2026-05-18T12:26:17Z"},{"alias_kind":"pith_short_8","alias_value":"ZJ3P46E3","created_at":"2026-05-18T12:26:17Z"}],"graph_snapshots":[{"event_id":"sha256:69d3a1e3e284a60919d2d9447332cf8df3f8dc34efec3371adc439e7081d3958","target":"graph","created_at":"2026-05-18T04:32:55Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"Protein footprinting is a new methodology that is based on probing, typically with the use of mass spectrometry, of reactivity of different aminoacid residues to a modifying reagent. Data thus obtained allow one to make inferences about protein conformations and their intermolecular interactions. Most of the protein footprinting studies so far have been performed on individual proteins in vitro. We explore whether a similar approach is possible with the proteins inside of living cells, employing dimethylsulfate (DMS), a reagent widely used for the in vivo footprinting of nucleic acids. DMS can","authors_text":"Arman Kulyyassov, Damien Vertut, Erlan Ramankulov, Luc Camoin, Marc Lipinski, Pasquale Moio, Vasily Ogryzko","cross_cats":[],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2010-12-20T12:58:55Z","title":"Exploring dimethylsulfate for in vivo proteome footprinting"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1012.4313","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:5fba30d2485d130ce1c93d9bf687985f7fcffe8b8b3cbbe9311ed382e0971804","target":"record","created_at":"2026-05-18T04:32:55Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"1cdb862e2ec31e9a0e7d4192680193a714eaf88bbbf47728ac06c22288e7770b","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2010-12-20T12:58:55Z","title_canon_sha256":"502dbcde5e5bc74f92bc21af1923258c1f5e7214edcd6eeac0036ef757c8b94c"},"schema_version":"1.0","source":{"id":"1012.4313","kind":"arxiv","version":1}},"canonical_sha256":"ca76fe789b07c2bece0316ac8bd81944c1a5e9eec2002318e14b192b0f1140bf","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"ca76fe789b07c2bece0316ac8bd81944c1a5e9eec2002318e14b192b0f1140bf","first_computed_at":"2026-05-18T04:32:55.032054Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T04:32:55.032054Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"eYVrqwVGGkCyo11LoiuPEmBEZ/dmPmgeE4c+Nn9InB58J+dj0jI7ilfMzwdurunIGx/puf872KinTrwLmLmNCA==","signature_status":"signed_v1","signed_at":"2026-05-18T04:32:55.032751Z","signed_message":"canonical_sha256_bytes"},"source_id":"1012.4313","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:5fba30d2485d130ce1c93d9bf687985f7fcffe8b8b3cbbe9311ed382e0971804","sha256:69d3a1e3e284a60919d2d9447332cf8df3f8dc34efec3371adc439e7081d3958"],"state_sha256":"a5c9d696b2a39d943b350e183f71db6b758391bd01bf04fc7aa6fe9997c992e5"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"fERCgf4hSVnjdBQDukwFOF/DBs1qH8j3OZo/CWynnkQLZ1ABBbyKzIR4kd4+0uDvQSbOkNIxGIr0tC/UuA6bDg==","signed_message":"bundle_sha256_bytes","signed_at":"2026-07-02T13:22:59.530657Z","bundle_sha256":"6f2bb332e34181bb2b7548d4cdb78786aa8eca691661a50ebe0525fbf87e7c08"}}