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arxiv 2209.01104 v1 pith:BQ3WZCHJ submitted 2022-09-02 physics.med-ph

Online calibration of a linear micro tomosynthesis scanner

classification physics.med-ph
keywords calibrationstagelayerlinearmicroonlineresolutionscanner
verification ladder T0 review T1 audit T2 compute T3 formal T4 reserved
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In a linear tomosynthesis scanner designed for imaging histologic samples of several centimeter size at 10 micrometer resolution, the mechanical instability of the scanning stage (+/-10 micrometers) exceeded the resolution of the image system, making it necessary to determine the trajectory of the stage for each scan to avoid blurring and artifacts in the images that would arise from the errors in the geometric information used in 3D reconstruction. We present a method for online calibration by attaching a layer of randomly dispersed micro glass beads or calcium particles to the bottom of the sample stage. The marker layer was easy to produce and proven effective in the calibration procedure.

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