Air cold atmospheric plasma with patterns for anaplastic squamous cell carcinoma treatment

In recent years, cold atmospheric plasma (CAP) using inert gas has been successfully applied for biomedicine, such as sterilization, wound healing, skin diseases, and tumor treatment. Here, we reported air cold atmospheric plasma with three different patterns (I. Non: basic square grid structure; II. Square: basic square grid structure + square node; III. Circle: basic square grid structure + circle node) for anaplastic squamous cell carcinoma treatment (VX2 cell line). Various plasma diagnostic techniques were applied to evaluate the physics of air CAP with patterns such as discharge voltage, plasma initial generating process, plasma temperature, and optical emission spectroscopy (OES). The direct effects of air CAP with patterns on anaplastic squamous cell carcinoma treatment (VX2 cell line) were investigated in vitro. We also studied the ROS (reactive oxygen species) and RNS (reactive nitrogen species) generation in cultured media released from VX2 cells after the treatment of air CAP with patterns. The results showed that the air CAP with circle-pattern generated more active substances during at 60s treatment time, which resulted in a higher death rate of VX2 cells. These initial observations establish the air CAP with patterns as potential clinical applications for cancer therapy.